15 Sigma ’ s New Enhanced Avian RT - PCR Kit
نویسندگان
چکیده
Reverse transcriptase-PCR (RT-PCR) is a powerful tool for gene expression analysis at the RNA level, primarily due to its sensitivity, speed, ease of use, and versatility. In a typical RT-PCR procedure, a reverse transcriptase is used to generate a complementary DNA (cDNA) copy of RNA molecules, followed by polymerase chain reaction (PCR) to amplify the cDNA. Since the PCR process part is usually robust and generic, performance of a given RT-PCR system is mainly determined by the activity of the reverse transcriptase used.
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